Thermal Stability of siRNA Modulates Aptamer- conjugated siRNA Inhibition
نویسندگان
چکیده
منابع مشابه
Aptamer mediated siRNA delivery
Nucleic acids that bind to cells and are subsequently internalized could prove to be novel delivery reagents. An anti-prostate specific membrane antigen aptamer that has previously been shown to bind to prostate tumor cells was coupled to siRNAs via a modular streptavidin bridge. The resulting conjugates could be simply added onto cells without any further preparation, and were taken up within ...
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During RNA-induced silencing complex (RISC) assembly the guide (or antisense) strand has to separate from its complementary passenger (or sense) strand to generate the active RISC complex. Although this process was found to be facilitated through sense strand cleavage, there is evidence for an alternate mechanism, in which the strands are dissociated without prior cleavage. Here we show that th...
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ژورنال
عنوان ژورنال: Molecular Therapy - Nucleic Acids
سال: 2012
ISSN: 2162-2531
DOI: 10.1038/mtna.2012.41